Department of Mocrobiology
MBiotec Group

WZW

Contents

Reviews

A.
Genetics in Gram-positive Bacteria

B.
Cellulase-Genes and Characterization of their Gene Products Ba. Cellulase-genes of Clostridium Thermocellum Bb. Cellulase Genes of Clostridium stercorarium Bc. Cellulase-Genes of other Bacteria
C. Other hydrolytic Enzyms - Genes and their Gene Products Ca. ß-Glucanases Cb. ß-Glucosidases and Cellodextrin-Phosphorylases Cc. ß-Xylanases and other Hydrolases for Xylan-Hydrolysis Cd. Hydrolases for Starch Hydrolysis Ce. Other hydrolases
D. Purification and Characterization of Cellulolytic Proteins

E.
Methodical Papers

F.
Bacterial Fermentations

G.
Rumen microbiology


Included are also the thesis titles (Diplom- und Doktorarbeiten) from 1991 onwards.

 

Reviews

Schwarz, W. H. (2001). The cellulosome and cellulose degradation by anaerobic bacteria. Appl. Microbiol. Biotechnol. 56:635-649. abstract

Schwarz, W. H. (2003). Das Cellulosom - eine Nano-Maschine zum Abbau von Cellulose. Naturwissenschaftliche Rundschau 56: 121-128.

Schwarz, W. H. (2003). The cellulases and their application in degrading agro-industrial waste (Las cellulasa y su aplicación en la degradación de desechos agroindustriales). Revista Colombiana de Biotecnología 4:6-13.

Schwarz, W. H. (2004). Cellulose - Struktur ohne Ende. Naturwissenschaftliche Rundschau 8:443-445.

Zverlov, V. V., & Schwarz, W. H. (2004). The Clostridium thermocellum cellulosome - the paradigm of a multienzyme complex. In Ohmiya, K., K. Sakka, S. Karita, T. Kimura, M. Sakka, and Y. Onishi (eds.), Proceedings of the Mie Bioforum 2003: Biotechnology of Lignocellulose Degradation and Biomass Utilization. Uni Pub. Co. Ltd., Tokyo. pp. 137-147.

Schwarz, W. H., Zverlov, V. V., & Bahl, H. (2004). Extracellular glycosyl hydrolases from clostridia. Adv. Appl. Microbiol., 56:215-261. abstract

Berezina, O., Zverlov, V. V., Velikodvorskaya, G. A., & Schwarz, W. H. (2006) Bacterial acetone and butanol production by industrial fermentation in the Union: use of hydrolyzed agricultural waste for biorefinery. Appl. Microbiol. Biotechnol. 71: 587-597. abstract

Schwarz, W. H., Zverlov, V. V. (2006). Protease inhibitors in bacteria: an emerging concept for the regulation of bacterial protein complexes? Molec. Microbiol. 60: 1323-1326 (doi: 10.1111/j.1365-2958.2006.05181.x) abstract

Schwarz, WH (2006) Nachwachsende Kraftstoffe. Naturwissenschaftliche Rundschau, 693: 151-154. ISSN 0028-1050.

Schwarz, W. H. & Gapes, J. R. (2006) Butanol - rediscovering a renewable fuel. BioWorld Europe 01-2006: 16-19. abstract

Zverlov, V. V., & Schwarz, W. H. (2006). The C. thermocellum cellulosome: novel components and insights from the genomic sequence. In Cellulosome, chapt. VI, ed. Vladimir Uversky & Irina Kataeva. (In: Molecular Anatomy and Physiology of Proteins, edited by Vladimir Uversky). Nova Publishers Kataeva, I., & Ljungdahl, L. (eds.). ISBN 1-59454-950-8.

Schwarz, W.H., Slattery, M., & Gapes J.R. (2007). The ABC of ABE. BioWorld Europe 02-2007: 8-10. abstract

A. Genetics in Gram-positive Bacteria

 

Luczak, H., Schwarzmoser and W.L. Staudenbauer (1985). Construction of a Clostridium butyricum hybrid plasmid and transfer to Bacillus subtilis. Appl. Microbiol. Biotechnol. 23:114-122.

Soutschek-Bauer E., L., Hartl, and W.L. Staudenbauer (1985). Transformation of Clostridium thermohydrosulfuricum DSM 568 with plasmid DNA. Biotechnol. Let. 7:705-710.

Schwarz, W.H., S. Schimming, and W.L. Staudenbauer (1987). High-level expression of Clostridium thermocellum cellulase genes in Escherichia coli. Appl. Microbiol. Biotechnol. 27: 50-56

Soutschek-Bauer, E., and W.L. Staudenbauer (1987). Synthesis and secretion of a heat-stable carboxymethylcellulase from Clostridium thermocellum in Bacillus subtilis and Bacillus stearothermophilus. Mol. Gen. Genet. 208 (1987) 537-541. abstract

Soutschek-Bauer, E. W. Scholz, E. Grill, and W.L. Staudenbauer (1987). Thermostability and superhelicity of plasmid DNA in Bacillus stearothermophilus. Mol. Gen. Genet. 20: 575-579. abstract

Dubbert, W. (1988). Genklonierung und Charakterisierung zweier Chloramphenicol-Acetyltransferasen aus Clostridium butyricum. Dissertation TUM.

Dubbert, W., H. Luczak, and W.L. Staudenbauer (1988). Cloning of two chloramphenicol acetyltransferase genes from Clostridium butyricum and their expression in Escherichia coli and Bacillus subtilis. Mol Gen. Genet. 214: 328-332. abstract

Young, M., Minton N.P., Staudenbauer W.L. (1989). Recent advances in the genetics of the clostridia. FEMS Microbiol. Rev. 63: 301-326. abstract

Landmann, B. (1991). Physikalische Charakterisierung des Genoms thermophiler Clostridien mit Hilfe der Pulsed-Field Agarose Gel-Elektrophorese. Diplomarbeit TUM.

Schulte-Spechtel, U. (1991). Entwicklung von Wirts-Vektro-Systemen für Genklonierungen in Clostridien. Dissertation TUM

Schwarz, W.H., B. Landmann, and W.L. Staudenbauer (1991). Use of pulsed-field agarose gele electrophoresis (PFGE) to compare genoms of thermophilic, cellulolytic clostridia. Electrophorese forum ´91, ed. B. J. Radola, München, Germany, p. 74-79

Schwarz, W.H., K. Bronnenmeier, B. Landmann, G. Wanner, W.L. Staudenbauer, N. Kurose, and T. Takayama (1995). Molecular characterization of four strains of the cellulolytic thermophile Clostridium stercorarium. Biosci. Biotech. Biochem. 9: 1661-1665

Zverlov, V.V., and Schwarz, W.H. (1999). Organization of the chromosomal region containing the genes lexA and topA in Thermotoga neapolitana. Primary structure of LexA reveals phylogenetic relevance. Syst. Appl. Microbiol. 22:174-178 abstract

 

B. Cellulase-Genes and Characterization of their Gene Products
 

Ba. Cellulase-Genes of Clostridium thermocellum

Schwarz, W., K. Bronnenmeier, and W. L. Staudenbauer (1985). Molecular cloning of Clostridium thermocellum genes involved in ß-glucan degradation in bacteriophage lambda. Biotechnol. Lett. 7: 859-864

Schwarz, W. H., F. Gräbnitz, and W. L. Staudenbauer (1986). Properties of a Clostridium thermocellum endoglucanase produced Escherichia coli. Appl. Environ. Microbiol. 51: 1293-1299

Schwarz, W. H., S. Schimming, K. P. Rücknagel, S. Burgschwaiger, G. Kreil, and W. L. Staudenbauer (1988). Nucleotide sequence of the celC gene encoding endoglucanase C of Clostridium thermocellum. Gene 63: 23-30. abstract

Schwarz, W.H., S. Schimming, and W.L. Staudenbauer (1988). Degradation of barley ß-glucan by endoglucanase C of Clostridium thermocellum. Appl. Microbiol. Biotechnol. 29: 25-31.

Zverlov, V.V., Velikodvorskaya, G.A., Schwarz, W.H., Bronnenmeier, K., Kellermann, J., and Staudenbauer, W.L. (1998). Multidomain structure and cellulosomal localization of the Clostridium thermocellum cellobiohydrolase CbhA. J. Bacteriol. 180:3091-3099. abstract

Schwarz, W.H. (1995). Hydrolysis of hemicellulose by thermostable recombinant enzymes derived from the thermophilic, anaerobic bacteria Clostridium thermocellum and Clostridium stercorarium. International Workshop "High-Temperature Biotechnology", Petropavlovsk-Kamchatsky, Russia.

Zverlov, V.V., Velikodvorskaya, G.A., Schwarz, W.H., Kellermann, J., and Staudenbauer, W.L. (1999). Duplicated Clostridium thermocellum cellobiohydrolase gene encoding cellulosomal subunits S3 and S5. Appl. Microbiol. Biotechnol. 51:852-859. abstract

Schwarz, W. H., and Zverlov, V. V. (2001). New components in the cellulosome of Clostridium thermocellum. Gordon Research Conference on "Cellulases and Cellulosomes", Procter Academy, Andover NH, USA, July 29-Aug. 3.

Zverlov V.V., Velikodvorskaya, G.A., and Schwarz, W.H. (2002). New cellobiohydrolase Cel5O in the cellulosome of Clostridium thermocellum: binding to cellulose and indication for processive hydrolysis of the substrate from the reducing end. Microbiol. 148: 247-255. abstract

Zverlov, V. V., Velikodvorskaya, G. A., Schwarz, W. H. (2003). Two new cellulosome components encoded downstream of celI in the genome of Clostridium thermocellum: the non-processive endoglucanase CelN and the possibly structural protein CseP. Microbiol. 149, 515-524. abstract

Zverlov, V. V., & Schwarz, W. H. (2004). The Clostridium thermocellum cellulosome - the paradigm of a multienzyme complex. In Ohmiya, K., K. Sakka, S. Karita, T. Kimura, M. Sakka, and Y. Onishi (eds.), Proceedings of the Mie Bioforum 2003: Biotechnology of Lignocellulose Degradation and Biomass Utilization. Uni Pub. Co. Ltd., Tokyo. pp. 137-147.

Zverlov, V. V., Kellermann, J., & Schwarz, W. H. (2005). Functional subgenomis of Clostridium thermocellum cellulosomal genes: Identification of the major components in the extracellular complex and detection of three new components. Proteomics 5: 3646-3653. abstract

Zverlov, V. V., Schantz, N., & Schwarz, W. H. (2005). A major new component in the cellulosome of Clostridium thermocellum is a processive endo-ß-1,4-glucanase producing cellotetraose. FEMS Microbiol. Let. 249: 353-358. abstract

Berger, E., Zhang, D., Zverlov, V. V. & Schwarz, W. H. (2007). Two noncellulosomal cellulases of Clostridium thermocellum, Cel9I and Cel48Y, hydrolyse crystalline cellulose synergistically. FEMS Microbiol. Let. 268: 194-201. abstract

 

Bb. Cellulase-Genes of Clostridium stercorarium

Schwarz, W.H., Jauris, S., Kouba, M., Bronnenmeier, K., and Staudenbauer, W.L. (1989). Cloning and expression of Clostridium stercorarium genes in Escherichia coli. Biotech. Let. 11: 461-466.

Jauris, S., Rücknagel, K.P., Schwarz, W.H., Kratzsch, P., Bronnenmeier, K. Staudenbauer, W.L. (1990). Sequence analysis of the Clostridium stercorarium celZ gene encoding a thermoactive cellulase (Avicelase I): Identification of catalytic and cellulose-binding domains. MGG 223: 258-267. abstract

Jauris, S. (1990). Klonierung und Sequenzierung von Genen cellulolytischer Enzyme aus Clostridium stercorarium. Dissertation TUM.

Bronnenmeier, K., Rücknagel, K.P., and Staudenbauer, W.L. (1991). Purification and properties of a novel type of exo-1,4-ß-glucanase (Avicelase II) from the cellulolytic thermophile Clostridium stercorarium. Eur. J. Biochem 200: 379-385. abstract

Kunth, K. (1991). Klonierung und Sequenzierung des Exoglucanasegens celY aus Clostridium stercorarium. Diplomarbeit TUM.

Ganghofner, D. (1991). Expression des Clostridium stercorarium Cellulase-Gens celZ in Bacillus subtilis und Charakterisierung des Genproduktes (Avicelase I). Diplomarbeit TUM.

Bauer, S. (1992). PCR-Deletionsanalyse der Cellulose-Bindungsregion der Cellulase CelZ (Avicelase I) aus Clostridium stercorarium. Diplomarbeit TUM.

Stingele, F. (1992). Konstruktion und Expression des celY Gens aus C. stercorarium in E. coli und B. subtilis und Charakterisierung des rekombinanten Genprodukts (Avicelase II). Diplomarbeit TUM.

Riedel, K., K. Bronnenmeier, K. Kunth, W. Schwarz, F. Stingele and W.L. Staudenbauer (1995). Clostridium stercorarium cellulase CelY: DNA sequence of the gene and characterization of the recombinant protein (Avicelase II). VAAM-Tagung Stuttgart.

Bronnenmeier, K., K. Kundt, K. Riedel, W.H. Schwarz, and W.L. Staudenbauer (1997). Structure of the Clostridium stercorarium gene celY encoding the exo-1,4-ß-glucanase Avicelase II. Microbiology 143:891-898. abstract

Riedel, K., Ritter, J., and Bronnenmeier, K. (1997) Synergistic interaction of the Clostridium stercorarium cellulases Avicelase I (CelZ) and Avicelase II (CelY) in the degradation of microcrystalline cellulose. FEMS Microbiol. Lett. 147:239-243.

Riedel K., and K. Bronnenmeier (1998). Intramolecular synergism in an engineered exo-endo-1,4-ß-glucanase fusion protein. Molec. Microbiol. 28:767-775. abstract

Riedel, K., and K. Bronnenmeier (1998). Intramolecular synergism in natural and engineered endo-exo-1,4-ß-glucanase fusion proteins, pp C75-78, Proceedings of he 7th International Conference on Biotechnology in the Pulp and Paper Industry, Vancouver.

Riedel, K., Ritter, J., Bauer, S., and Bronnenmeier, K. (1998) The modular cellulase CelZ of the thermophilic bacterium Clostridium stercorarium contains a thermostabilizing domain. FEMS Microbiol. Let. 164:261-267. abstract

Riedel, K., and K. Bronnenmeier (1998) Intramolecular synergism in natural and engineered endo-exo-1,4-ß-glucanase fusion proteins, pp C75-78, Proceedings of he 7th International Conference on Biotechnology in the Pulp and Paper Industry, Vancouver.

Riedel K, Bronnenmeier K. (1999). Active-site mutations which change the substrate specificity of the Clostridium stercorarium cellulase CelZ implications for synergism. Eur J Biochem 1999 May;262(1):218-23. abstract

 

Bc. Cellulase-Genes of other Bacteria

Wachinger, G., Bronnenmeier, K., Staudenbauer, W.L., and Schrempf, H. (1989) Identification of mycelium-associated cellulase from Streptomyces reticuli. Appl. Env. Microbiol. 55:2653-2657.

Bronnenmeier, K., A. Kern, W. Liebl, W.L. Staudenbauer (1995). Purification of Thermotoga maritima enzymes for the degradation of cellulosic materials. Appl. Environm. Microbiol. 61:1399-1407. abstract

Liebl W., P. Ruile, K. Bronnenmeier, K. Riedel, F. Lottspeich, and I. Greif. (1996). Analysis of a Thermotoga maritima DNA fragment encoding two similar thermostable cellulases, CelA and CelB, and characterization of the recombinant enzymes. Microbiology 142:2533-2542. abstract

Zverlov, V.V., and Bronnenmeier, K. (1997). Sequencing of endoglucanase celD and endoxylanase xynA genes from the extremely thermophilic bacterium Anaerocellum thermophilum Z-1320. VAAM meeting, Hamburg. BIOspectrum 1997, p. 52.

Zverlov, V.V., Mahr S, Riedel, K., and Bronnenmeier, K. (1998). Properties and gene structure of a bifunctional cellulolytic enzyme (CelA) from the extreme thermophile "Anaerocellum thermophilum" with separate glycosyl hydrolase family 9 and 48 catalytic domains. Microbiology 144:457-465. abstract

Vargas, L.C., Montoya, D., & Aristizábal, F. (2002). Clonación y expresión en Escherichia coli de genes de celulase de Clostridium IBUN 22A. Revista Colombiana de Biotechnología 4: 29-35.

 

 

C. Other hydrolytic Enzyms - Genes and their Gene Products

Ca. ß-Glucanases

Schwarz, W.H., S. Schimming, and W.L. Staudenbauer (1988). Isolation of a Clostridium thermocellum gene encoding a thermostable ß-1,3-glucanase (laminarinase). Biotechnol. Let. 10: 225-230.

Schimming, S., Schwarz, W.H., and Staudenbauer, W.L. (1991). Properties of a thermoactive ß-1,3-1,4-glucanase (lichenase) from Clostridium thermocellum expressed in Escherichia coli. BBRC 177: 447-452. abstract

Schimming, S. (1991). Struktur der ß-Glucanase Gene licA und licB aus Clostridium thermocellum und Charakterisierung der Genprodukte. Dissertation TUM.

Schimming, S., W.H. Schwarz, and W.L. Staudenbauer (1992). Structure of the Clostridium thermocellum gene licB and the encoded ß-1,3-1,4-glucanase. A catalytic region homologous to Bacillus lichenases joined to the reiterated domain of clostridial cellulases. Eur. J. Biochem. 204: 13-19. abstract

Riedel, K. (1994). Isolierung, Reinigung und Charakterisierung der katalytischen Domäne der rekombinanten ß-Glukanase LicA aus Clostridium thermocellum. Diplomarbeit TUM.

Zverlov, V. V., K.P. Fuchs, W.H. Schwarz, and G.A. Velikodvorskaya (1994). Purification and cellulosomal localization of Clostridium thermocellum mixed linkage ß-glucanase LicB (1,3-1,4-ß-D-glucanase). Biotechnol. Let. 16: 29-34.

Fuchs, K.P., W. H. Schwarz, and W.L. Staudenbauer (1995). Highly active ß-glucanase LicB from the thermophilic anaerobic bacterium Clostridium thermocellum. VAAM-Meeting Stuttgart.

Bauer, M. S. (1995). Untersuchungen über die heterologe Expression der ß-1,3-glucanase LicA aus Clostridium thermocellum in Bacillus-Stämmen. Diplomarbeit TUM.

Zverlov, V.V., and Schwarz, W.H. (1997). Nucleotide sequence of the laminarinase gene from Thermotoga neapolitana and characterization of the recombinant gene product. VAAM meeting, Hamburg. BIOspectrum 1997, p. 51.

Fuchs, K.P., and Schwarz, W.H. (1997.) Porperties of the cellassociated thermostable ß-1,3(4)-glucanase LicA from Clostridium thermocellum. VAAM meeting, Hamburg. BIOspektrum 1997, p. 101.

Zverlov, V.V., I.Y. Volkov, T.V. Velikodvorskaya, and W.H. Schwarz (1997). Highly thermostable endo-1,3-ß-glucanase (laminarinase) LamA from Thermotoga neapolitana: Nucleotide sequence of the gene and characterization of the recombinant gene product. Microbiology 143:1701-1708. abstract

Fuchs, K.P. (1997). Molekularbiologische Charakterisierung der ß-Glukanasen LicA und LicB aus Clostridium thermocellum. Shaker-Verlag / Aachen, ISBN 8265-2275-3.

Fuchs, K.P. (1997). Molekularbiologische Charakterisierung der ß-Glukanasen LicA und LicB aus Clostridium thermocellum. Dissertation TUM

Zverlov, V.V., and Schwarz, W. H. (2000). The binding specificity of two Thermotoga neapolitana laminarinase A substrate binding domains. VAAM Meeting "Microbiology 2000", München. BIOspektrum 2000, Sonderausgabe, p. 74.

Zverlov, V.V., Volkov, I.Y., Velikodvorskaya, G.A., and Schwarz, W.H. (2001). The binding pattern of two carbohydrate binding modules of laminarinase Lam16A from Thermotoga neapolitana: differences in ß-glucan binding within family CBM4. Microbiology 147: 621-629. abstract

Fuchs, K. P., Zverlov, V. V., Velikodvorskaya, G. A., Lottspeich, F., and Schwarz, W. H. (2003). Lic16A of Clostridium thermocellum, a non-cellulosomal, highly complex endo-ß-1,3-glucanase bound to the outer cell surface. Microbiology 149: 1021-1031. abstract

 

Cb. Glycosyl-Hydrolases and -Phosphorylases

Gräbnitz, F., K.R. Rücknagel, M. Seiß, and W.L. Staudenbauer (1989). Nucleotide sequence of the Clostridium thermocellum bglB gene encoding thermostable ß-glucosidase B: Homology to fungal ß-glucosidases. Mol. Gen. Genet. 217: 70-76. abstract

Gräbnitz, F. (1989). Nukleotidsequenzen der ß-Glucosidase Gene bglA und bglB aus Clostridium thermocellum und Charakterisierung der Genprodukte. Dissertation TUM.

Gräbnitz, F., Seiß, M., Rücknagel, K.P., and Staudenbauer, W.L. (1991). Structure of the Clostridium thermocellum bglA gene coding for ß-glucosidase A. Sequence analysis reveals a superfamily of cellulases and ß-glucosidases including human lactase/phlorizin hydrolase. Eur. J. Biochem. 200: 301-310. abstract

Zverlov, V.V., Volkov, I.Y., Velikodvorskaya, T.V., and Schwarz, W.H. (1997). Thermotoga neapolitana bglB gene, upstream of lamA, encodes a highly thermostable ß-glucosidase that is a laminaribiase. Microbiology 143:3537-3542. abstract

Reichenberger, M., Lottspeich, F., and Bronnenmeier, K. (1997). Purification and properties of a cellobiose phosphorylase (CepA) and a cellodextrin phosphorylase (CepB) from the cellulolytic thermophile Clostridium stercorarium. Eur. J. Biochem. 247:262.267. abstract

Breves, R., Bronnenmeier, K., Wild, N., Lottspeich, F., Staudenbauer, W.L., and Hofemeister, J. (1997) Genes encoding two different ß-glucosidases of Thermoanaerobacter brockii are clustered in a common operon. Appl. Environ. Microbiol. 63:3902-3910. abstract

 

Cc. ß-Xylanases and other Hydrolases for Xylan Hydrolysis

Schwarz, W.H., Adelsberger, H., Jauris, S., Hertel, C., Funk, B., and Staudenbauer, W.L. (1990). Xylan degradation by the thermophile Clostridium stercorarium: Cloning and expression of xylanase, ß-xylosidase and alpha-L-arabinofuranosidase genes in Escherichia coli. BBRC 170: 368-374. abstract

Krause, B. (1991). Genklonierung und Charakterisierung zweier hemicellulolytischer Enzyme aus Clostridium stercorarium. Diplomarbeit TUM.

Adelsberger, H. (1992). Das Hemicellulase-System von Clostridium stercorarium: Struktur und Expression des Xylanase-Gens xynA und der ß-Xylosidase-Gene bxlA und bxlB, sowie Charakterisierung der rekombinanten Genprodukte. Dissertation TUM.

Vilbig A. G. (1992). Sequenzierung des alpha-Arabinofuranosidase-Gens arfA aus Clostridium stercorarium mit dem nicht-radioaktiven Direkt-Blotting-Elektrophorese-System. Diplomarbeit TUM.

Glawischnig, E. (1993). Struktur und Funktion der "xynB"-Region des Genoms von Clostridium stercorarium: Sequenzierung der Gene xynB, uxbA, uxbB und Charakterisierung der gereinigten rekombinanten Xylanase XynB. Diplomarbeit TUM.

Plitz, T. M. (1993). Isolierung des Gens xynZ aus einer Thermoanaerobacterium saccharolyticum B6A Genbank sowie Reinigung und Charakterisierung einer Xylanase (XynZ) mit neuartigen Eigenschaften. Diplomarbeit TUM.

Heidenthal (Sandnes), A. K. (1994). Klonierung, Expression, Reinigung und Charakterisierung der katalytischen Domäne der rekombinanten Celloxylanase CelX von Clostridium stercorarium. Diplomarbeit TUM.

Bronnenmeier K., Meissner H., Stocker S., and Staudenbauer W.L. (1995). alpha-D-glucuronidases from the xylanolytic thermophiles Clostridium stercorarium and Thermoanaerobium saccharolyticum. Microbiology 141: 2033-2040. abstract

Schwarz, W.H., K. Bronnenmeier, B. Krause, F. Lottspeich, and W.L. Staudenbauer (1995). Debranching of arabinoxylan: Properties of the thermoactive recombinant a-L-arabinofuranosidase from Clostridium stercorarium (ArfB). Appl. Microbiol. Biotechnol. 43:856-860. abstract

Schwarz, W.H. (1995). Complete hydrolysis of arabinoxylan by thermostable recombinant enzymes of the anaerobic bacterium Clostridium stercorarium. VAAM-meeting Stuttgart

Zverlov, V.V., Liebl, W., Bachleitner, M., and Schwarz, W.H. (1998). Nucleotide sequence of arfB of Clostridium stercorarium, and prediction of catalytic residues of a-L-arabinofuranosidase based on local similarity with several families of glycosyl hydrolases. FEMS Microbiol. Lett. 164:337-343. abstract

Donaghy, JA, Bronnenmeier K, Soto-Kelly PF, McKay AM (1999). Purification and characterization of an extracellular feruloyl esterase from the thermophilic anaerobe Clostridium stercorarium. J. Appl. Microbiol. 88:458-466. abstract

Zverlov, V.V., Hertel, C., Bronnenmeier, K., Hroch, A., Kellermann, J., and Schwarz, W.H. (1999). The thermostable a-L-rhamnosidase RamA of Clostridium stercorarium: biochemical characterization and primary structure of a bacterial alpha-L-rhamnoside hydrolase, a new type of inverting glycosyl hydrolase. Molec. Microbiol. 35:173-179. abstract

Adelsberger, H., Hertel, C., Glawischnig, E., Zverlov, V.V., and Schwarz, W.H. (2004). Enzyme system of Clostridium stercorarium for hydrolysis of arabinoxylan: reconstitution of the in vivo system from recombinant enzymes. Microbiol. 150:2257-2266. abstract

Zverlov, V. V., Schmitt-Kopplin, P., Schantz, N., & Schwarz, W. H. (2005). Two new major subunits in the cellulosome of Clostridium thermocellum: xyloglucan hydrolase Xgh74A and endo-xylanase Xyn10D. Microbiol. 151: 3395-3401. ISSN 1350-0872. Copyright Society for General Microbiology, Reading, UK. abstract

 

Cd. Hydrolases for Starch Hydrolysis

Huber, W. (1991). Isolierung und Charakterisierung thermoaktiver Amylopullulanasen aus Clostridium thermohydrosulfuricum. Diplomarbeit TUM.

Ritter, J. (1992). Reinigung und Charakterisierung zweier thermostabiler Glucoamylasen aus Clostridium thermohydrosulfuricum. Diplomarbeit TUM.

Müller, C. (1994). Reinigung einer Glucoamylase aus Thermoanaerobacter thermohydrosulfuricus. Diplomarbeit TUM

Wimmer, B. (1994). Reinigung und Charakterisierung einer alpha-Glukosidase/Maltodextrinhydrolase und einer Glucoamylase aus Thermoanaerobacter thermohydrosulfuricus. Diplomarbeit TUM.

Ganghofner, Dirk. Das amylolytische Enzymsystem von Clostridium thermosaccharolyticum: Reinigung und Charakterisierung einer Glukoamylase, einer Amylopullulanase und einer Isomaltase. Dissertation TUM, 1995.

Wimmer, B., Lottspeich, F., Ritter, J., and Bronnenmeier, K. (1997) A novel type of thermostable alpha-D-glucosidase from Thermoanaerobacter thermohydrosulfuricus exhibiting maltodextrinohydrolase activity. Biochem. J. 328:581-586. abstract

Ganghofner, D., Kellermann, J., Staudenbauer, W.L., and Bronnenmeier, K. (1998). Purification and properties of an amylopullulanase, a glucoamylase, and an alpha-glucosidase in the amylolytic enzyme system of Thermoanaerobacterium thermosaccharolyticum. Biosci. Biotechnol. Biochem. 62:302-308. abstract

O. V. Berezina, N. A. Lunina, V. V. Zverlov, D. G. Naumoff, W. Liebl, G. A. Velikodvorskaya (2003) A cluster of Thermotoga neapolitana genes involved in the degradation of starch and maltodextrins: The molecular structure of locus. Mol. Biology 37: 678-685. [abstract]

N. A. Lunina, O. V. Berezina, B. Veith, V. V. Zverlov, I.P. Vorobieva, L. A. Chekanovskaya, I. S. Khromov, C. Raasch, W. Liebl, G.A.Velikodvorskaya (2003). A cluster of Thermotoga neapolitana genes involved in the degradation of starch and maltodextrins: the expression of the aglB and aglA genes in E. coli and the properties of the recombinant enzymes. Mol. Biology 37: 686-694. [abstract]

Veith, B., Zverlov, V. V., Lunina, N. A., Berezina, O. V., Raasch, C., Velikodvorskaya, G. A., and Liebl, W. (2003). Comparative analysis of the recombinant alpha-glucosidases from the Thermotoga neapolitana and Thermotoga maritima maltodextrin utilization gene clusters. Biocatalysis and Biotransformation 21: 147-158. [abstract]

 

Ce. Other hydrolases

Zverlov, V. V., Fuchs, K.-P., & Schwarz, W. H. (2002) Chi18A, the endochitinase in the cellulosome of the thermophilic, cellulolytic bacterium Clostridium thermocellum. Appl. Environ. Microbiol. 68: 3176-3179. abstract

 

D. Purification and Characterisation of Cellulolytic Proteins

Bronnenmeier, K., and W.L. Staudenbauer (1988). Resolution of Clostridium stercorarium cellulase by Fast Protein Liquid Chromatography (FPLC). Appl. Microbiol. Biotechnol. 27: 432-436.

Bronnenmeier, K., and W.L. Staudenbauer (1988). Purification and properties of an extracellular ß-glucosidase from the cellulolytic thermophile Clostridium stercorarium. Appl. Microbiol. Biotechnol. 28: 380-386.

Gräbnitz, F., and W.L. Staudenbauer (1988). Characterization of two ß-glucosidase genes from Clostridium thermocellum. Biotechnol. Lett. 10: 73-78.

Bronnenmeier, K., and Staudenbauer, W.L. (1990). Cellulose hydrolysis by a highly thermostable endo-1,4-ß-glucanase (Avicelase I) from Clostridium stercorarium. Enz. Microb. Technol. 12: 431-436.

Bronnenmeier, K., Ebenbichler, C., and Staudenbauer W.L. (1990). Separation of the cellulolytic and xylanolytic enzymes of Clostridium stercorarium. J. Chromatography 521: 301-310.

 

 

E. Methodical Papers

Schwarz, W.H., K. Bronnenmeier, F. Gräbnitz, and W.L. Staudenbauer (1987). Activity staining of cellulases in polyacrylamide gels containing mixed linkage ß-glucans. Anal. Biochem. 164: 72-77. abstract

Bronnenmeier, K., Adelsberger, H., Lottspeich, F., and Staudenbauer, W.L. (1996). Affinity purification of cellulose-binding enzymes of Clostridium stercorarium. Bioseparation 6:41-45. abstract

Zverlov, V. V., Höll, W., and Schwarz, W. H. (2003). Enzymes for digestion of cellulose and other polysaccharides in the gut of longhorn beetle larvae, Rhagium inquisitor L. (Col., Cerambycidae). Int. Biodet. Biodegr. 51:175-179. abstract

 

 

F. Bacterial Fermentations

Job, C., C. Schertler, W.L. Staudenbauer, and E. Blass (1989). Selection of organic solvents for in situ extraction of fermentation products of Clostridium thermohydrosulfuricum. Biotechnol. Techn. 3: 315-320

Mayer, M. (1992). Reinigung der Acetat-Kinase, Phosphotransacetylase und Lactatdehydrogenase von Clostridium thermohydrosulfuricum und Charakterisierung von Acetat-negativen Mutanten. Diplomarbeit TUM

Mayer, M., K. Bronnenmeier, W.H. Schwarz, C. Schertler, and W.L. Staudenbauer (1995). Isolation and properties of acetate kinase- and phosphotransacetylase-negative mutants of Thermoanaerobacter thermohydrosulfuricus. Microbiol. 141:2891-2896.

Schwarz, W. H. (2000). Isolation of new mesophilic solvent-producing Clostridia from Colombian soil: Taxonomical position, polysaccharide hydrolysis and solvent production. Clostridium 2000, The Sixth international workshop on regulation of metabolism, genetics, and development of the solvent and acid-forming Clostridia. UIUC, Urbana-Champaign, USA.

Montoya, D., Aristizabal, F., Gonzalez, S., Arévalo, C., and Schwarz, W.H. (2000). Caracterizacion por electroforesis de campo pulsado y 16S rRNA de cepas de Clostridium solventogenicos de origen colombiano. IV. Congreso Colombiano Genetica, 23-25. Febrero de 2000 in Popayan, Colombia.

Montoya, D., Spitia, S., Silva, E., and Schwarz, W.H. (2000). Isolation of mesophilic solvent-producing clostridia from Colombian sources: Physiological characterization, solvent production and polysaccharide hydrolysis. J. Biotechnol. 79:117-126. abstract

Montoya, D., Aristizabal, F., Perdomo, L., Arévalo, C., and Schwarz, W. (2000). Caracterizacion de cepas nativas de Clostridium ssp por secuencacion parcial del gen ribosomal 16S rARN. Revista Colombiana de Biotecnologia, Vol. II, 35-39.

Montoya, D., Arévalo, C., Aristizabal, F., and Schwarz, W.H. (2001). New solvent-producing Clostridium sp. strains, hydrolyzing a wide range of polysaccharides, are closely related to Clostridium butyricum. J. Ind. Microbiol. Biotechnol. 27:329-335. abstract

Montoya Castano, D. (2003). Anaerobic, solvent-producing bacteria: Molecular characterisation, polysaccharolytic activity and agro-industrial waste degradation. PhD thesis, Technical University Muenchen.

Schieder, D., Prechtl, S, Igelspacher, R., Antoni, D., Schwarz, W. H., Kroner, Th., Faulstich, M., Wagner, U., Meyer-Pittroff, R., Bauer, W., Staudenbauer, W. (2004). Bioethanol aus lignocellulosehaltiger Biomasse - Potenziale und Technologien, Tagungsband Kraftstoffe der Zukunft 2004, Bundesverband Bioenergie e.V., Bonn 2004.

Berezina, O., Zverlov, V. V., Velikodvorskaya, G. A., & Schwarz, W.H. (2006). Bioethanol from ligno-cellulosic biomass in the Soviet Union: use of Timisoara. Transcactions on Mechanics 51 (65), 297-302.

Igelspacher, R., Antoni, D., Kroner, T., Schwarz, W. H., Prechtl, S, Schieder, D. & Faulstich, M. (2006). Bioethanolproduktion aus Lignocellulose. Brennstoff Wärme Kraft BWK, VDI-Verlag, vol. 58 (3), p. 50-54. [abstract] ISSN: 1618-193X (Gedruckte Ausgabe), Springer-VDI-Verlag, Düsseldorf

Antoni, D., Igelspacher, R., Kroner, T., Prechtl, S., Schieder, D., Schwarz, W.H., & Faulstich, M. (2006). Bioethanol out of lignocellulosic biomass in Bavaria - a feasibility study. COFRET.

 

G. Rumen Microbiology and safety aspects

Einspanier, R., Lutz, B., Rief, S., Berezina, O., Zverlov, V., Schwarz, W., and Mayer, J. (2004). Tracing residual recombinant feed molecules during digestion and rumen bacterial diversity in cattle fed transgene maize. Eur. Food Res. Technol. 218: 269-273. abstract

Shedova, E., Albrecht, C., Zverlov, V.V., & Schwarz, W.H. (submitted) Stimulation of DNA transformation in Streptococcus gordonii by cattle saliva and native isolates.

 

last update: 26.05.2007